Insights into rubber biosynthesis from transcriptome analysis of Hevea brasiliensis latex
نویسندگان
چکیده
منابع مشابه
Insights into rubber biosynthesis from transcriptome analysis of Hevea brasiliensis latex.
Hevea brasiliensis is the most widely cultivated species for commercial production of natural rubber (cis-polyisoprene). In this study, 10,040 expressed sequence tags (ESTs) were generated from the latex of the rubber tree, which represents the cytoplasmic content of a single cell type, in order to analyse the latex transcription profile with emphasis on rubber biosynthesis-related genes. A tot...
متن کاملA Stimulator Protein of Rubber Biosynthesis from Hevea brasiliensis Latex
A rubber biosynthesis stimulator protein (RBSP)from the C-serum of Hevea brasiliensis latex was purified and characterised. This proteinaceous factor stimulates the incorporation of isopentenyl diphosphate into rubber and thereby enhances the rate of rubber biosynthesis. The heat stable RBSP is shown to have a molecular weight of 13 068 Da by mass spectroscopy. Amino acid sequence of peptide fr...
متن کاملMetabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex
The cytosolic mevalonate (MVA) pathway in Hevea brasiliensis latex is the conventionally accepted pathway which provides isopentenyl diphosphate (IPP) for cis-polyisoprene (rubber) biosynthesis. However, the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway may be an alternative source of IPP since its more recent discovery in plants. Quantitative RT-PCR (qRT-PCR) expression profiles ...
متن کاملThe biosynthesis of rubber. Incorporation of mevalonate and isopentenyl pyrophosphate into rubber by Hevea brasiliensis-latex fractions.
The position of mevalonic acid and isopentenyl pyrophosphate (3 methylbut 3 enyl pyrophosphate) as key intermediates in the biosynthesis of isoprenoid compounds is now firmly established, as also is the route by which mevalonic acid is converted into isopentenyl pyrophosphate. This transformation occurs through three ATP-dependent steps with the intermediate formation of mevalonate 5-phosphate ...
متن کاملDe Novo Assembly and Transcriptome Analysis of the Rubber Tree (Hevea brasiliensis) and SNP Markers Development for Rubber Biosynthesis Pathways
Hevea brasiliensis (Willd. Ex Adr. Juss.) Muell.-Arg. is the primary source of natural rubber that is native to the Amazon rainforest. The singular properties of natural rubber make it superior to and competitive with synthetic rubber for use in several applications. Here, we performed RNA sequencing (RNA-seq) of H. brasiliensis bark on the Illumina GAIIx platform, which generated 179,326,804 r...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2007
ISSN: 0022-0957,1460-2431
DOI: 10.1093/jxb/erm093